MMP2 and neoplasm: Esculetin was demonstrated to inhibit the migration of many tumor cells, including human laryngeal cancer Hep-2 cells,[52] cervical cancer HeLa cells,[55] prostate cancer PC3 cells,[51] etc. In human colorectal cancer HCT-116 cells, esculetin (20 μg/mL) alone significantly inhibited cell migration and had a synergistic effect when used in combination with 5-fluorouracil (10 μg/mL).[64] It was further found that tumor migration-related target molecules, MMP-2 and MMP-7, were significantly downregulated by esculetin treatment in HCT-116 cells.