mRNA expression of Opn was significantly upregulated by BioC and BC in Kusa-A1 cells compared with the negative control (DW) and AHP (P < 0.001, Fig. 1a), and the mRNA expression of Oc was significantly upregulated by WST in Kusa-A1 cells compared with the negative control (P < 0.01, Fig. 1a). This evidence concerns the gene SPP1 and breast cancer.