A further interrogation revealed three distinct TME immune profiles based on a hierarchical clustering of the tumor transcriptome data; (1) an immune active cluster (cluster 3), formed mostly by OS, and enriched in macrophages and CD8+ expression, (2) a cluster enriched in monocytes and with patients with SD response (cluster 4), and (3) a slightly immune desert cluster consisting of all ES patients except one (cluster 5). The gene discussed is CD8A; the disease is Ewing sarcoma.