Using public datasets GSE15781 and GSE50760 from NCBI-GEO (https://www.ncbi.nlm.nih.gov/geo/), we validated CLDN1, CLDN2, and CLDN14 to be significantly upregulated and CLDN8 and CLDN23 to be significantly downregulated in normal colon versus tumor tissues from human samples. The gene discussed is CLDN1; the disease is neoplasm.