CLTI, chronic limb-threatening ischemia; eGFR, estimated glomerular filtration rate; HbA1c, hemoglobin A1c; HFrEF, heart failure with reduced ejection fraction; IFCC, the International Federation of Clinical Chemistry and Laboratory Medicine; LVEF, left ventricular ejection fraction; SGLT2, sodium-glucose co-transporter 2. The gene discussed is SLC5A2; the disease is heart failure.