Kinetic analysis of TO-PRO-3 uptake in wild type (WT), gasdermin D-deficient, or caspase-1/11-deficient macrophages confirmed that gasdermin D and caspase-1 are required for TO-PRO-3 uptake in response to Salmonella infection (Fig. 1e and Sup Fig. 1a). Here, CASP1 is linked to Salmonella Infections.