Clusters 4, 10, 11, 13–16, 18, 20, and 21 were labeled as AML blast clusters based on the following factors: (i) substantial proportion cells in the clusters from AML samples (Supplementary Fig. 9a, c) (ii) lack of established normal cell marker expression like CD3D, CD14, etc. (Supplementary Fig. 9b), (iii) expression of known AML blast markers, i.e., CD38, MPO, and AZU1 (Supplementary Fig. 9b), and (iv) SingleR annotation of cell clusters as myeloid progenitors (GMP, CMP) (Supplementary Fig. 9c). The gene discussed is MPO; the disease is acute myeloid leukemia.