MHC-I expression increased in MCC13 and UISO, thelines with low pretreatment MHC I expression, when MCC cells transfectedwith 4-1BBL NPs or 4-1BBL/IL-12 NPs were cocultured with 5000 CD8+T cells, and when MCC cells were transfected with any therapeuticNPs and cocultured with 50,000 CD8+ T cells (Figure 3B). Here, CD8A is linked to Merkel cell skin cancer.