In pathway analysis, we illustrated that PRAME-positive gliomas possessed higher activity of Hedgehog, P3IK-AKT-mTOR, P53, apoptosis, IL2-STAT5, and Wnt/β-catenin signaling pathways and lower expression of E2F targets, G2M mitotic checkpoint, reactive oxidative oxygen species, TNF-α, IL6-JAK-STAT6, inflammatory response, angiogenesis, epithelial-mesenchymal transition, mTORC1, glycolysis, and hypoxia. Here, PRAME is linked to central nervous system cancer.