The results showed that 79 DEGs affected the prognosis of osteosarcoma, with PDE4C, CFAP44, CARNS1, GREB1L, ROF207 identified as significant risk factors and GBP1, MSC, GBP3, F13A1, CCL2 as substantial protective factors (Figure 3A). This evidence concerns the gene PDE4C and osteosarcoma.