ATF7IP and otitis media: A total of 31 additional strains were used from which nine were successfully transformed with at least one pTBH plasmid, including NTHi R2866 and 86-028NP otitis media isolates (42, 43), and the NTHi P589, P593, P597, P607, P610, P621, and P656 COPD isolates (16) (Table S1; Fig. S3).