PECAM1 and hydrops fetalis: To investigate these transcriptional changes in the current context, stromal vascular fraction cells were isolated from the visceral adipose tissue of endothelial lineage-traced mice that were fed HF/HS diet for 52 weeks, and cells were separated by FACS into CD31+ eYFP+ endothelial cells and CD31−eYFP+ EndoMT cells.