p0071 protein levels gradually decreased in L. interrogans- and L. biflexa-infected RPTECs until 12 h p.i. However, in L biflexa-infected RPTECs, p0071 levels were recovered to basal levels at 24 h p.i. In contrast, in L. interrogans-infected RPTECs, p0071 continuously decreased during the course of infection and was significantly decreased at 24 h p.i. (~ 30% of L. biflexa-infected RPTECs, p <0.01) (Figures 2A, B). The gene discussed is PKP4; the disease is infection.