To determine whether expression of p53 isoforms was linked to the development of metastasis or melanoma-specific survival, we determined the optimum cut-off using the Cutoff Finder application, which determined the optimal cut-off as the point with the most significant (log-rank test) split [26] and Kaplan–Meier analysis to assess the differences in metastasis development over time. This evidence concerns the gene TP53 and melanoma.