Additionally, pretreatment of B16-OVA cells with AC484 led to significantly more tumour killing and cytokine production (IFNγ and TNF) by untreated SIINFEKL-specific OT-I T cells in vitro (Fig. 2f and Extended Data Fig. 1g) than vehicle-treated cells, a result consistent with enhanced antigen presentation. The gene discussed is TNF; the disease is neoplasm.