Analysis conducted at day 20 after tumor cell inoculation (Fig. 4f) revealed that IV BCG did not reduce B16-F10 lung metastases in Batf3−/− mice, in contrast to WT mice (Fig. 4f), which correlated with a lower infiltration of CD8+ T cells in the lungs (Fig. 4g). This evidence concerns the gene CD8A and neoplasm.