This cytotoxicity was tumor-specific, as this effect was not observed when non-antigenically related LLC cells were used as targets (Fig. 3j) Furthermore, to validate our findings in vivo, we reconstituted Rag1−/− mice (lacking T and B cells) with splenocytes from mice bearing B16-F10 lung tumors, treated with either IV PBS or BCG, or with splenocytes from tumor-free mice immunized with IV BCG as a control. The gene discussed is RAG1; the disease is neoplasm.