In this report, given the importance of MDCK cells in laboratory isolation and cell-based production of influenza viruses, we engineered MDCK cells, and MDCK-derived SIAT and hCK cells, to overexpress an extension enzyme, B3GNT2, to enhance N-glycan extension (NExt) and assessed its impact on H3N2 virus HA binding and virus infection. Here, B3GNT2 is linked to viral infectious disease.