Dysregulation of the Wnt/β-catenin pathway is a hallmark of CRC (Krausova & Korinek, 2014; Basu et al, 2016); thus, its key downstream target genes (Axin2 and C-Myc) were studied at different time points in our control and with experimental MUC13 expression cell lines (SW480 versus SW620; SW480+Vec versus SW480+MUC13; SW620+Vec versus SW620+MUC13) using our anoikis model by qRT-PCR (Herbst et al, 2014; Dai et al, 2018; Liu et al, 2021). This evidence concerns the gene AXIN2 and colorectal carcinoma.