Our study defined three main points: (1) MUC13 forms a molecular complex with YAP1; (2) MUC13 promotes nuclear shuttling of YAP1/β-catenin survival complex and YAP1, and (3) MUC13 influences the expression of survival/metastasis-associated genes in cancer cells during the anchorage-independent survival period, providing anoikis resistance. This evidence concerns the gene YAP1 and cancer.