Tissue or body fluids (blood, urine, pleural effusion, etc.)have EGFR altered by NGS detection Sensitivity, with or without other gene mutations; Receiving EGFR-TKI; Viruses can be detected according to RECIST version 1.1 and regularly checked in our hospital; Adults (age ≥18 years old) are generally in good condition [17]. This evidence concerns the gene EGFR and Pleural effusion.