Given that monocyte differentiation is believed to proceed from classical CD14+CD16− to non-classical CD14−CD16+ monocytes via intermediate CD14+CD16+ monocytes 61, these results may suggest that monocytes in the tumor microenvironment post vaccination undergo increased differentiation from intermediate to non-classical subpopulations, thereby causing a general skewing away from classical monocytes. The gene discussed is CD14; the disease is neoplasm.