T cells were enriched from each patient’s post vaccine sample by negative selection and then stimulated with autologous immortalized normal B cells (as antigen presenting cells, APCs) transfected with either Ig VH and VL sequences (expressed as sFv’s) derived from the respective patient-specific tumor idiotype (used previously for therapeutic vaccine production), or HIV Nef as a negative control, described previously 52. Here, S100B is linked to neoplasm.