CD8A and neoplasm: To obtain further insights into how PD-1 inhibition impacts PKM2KO CD8 + T cells, we performed RNA sequencing (RNA-seq) analyses of sorted tumor-infiltrating T cells from HKP1-ova-GFP tumor-bearing mice adoptively co-transferred with a mix of PKM2KO and PKM2WT OT-I + T cells and treated with IgG control or anti-PD-1 (Fig. 4G, Extended Data Fig. 8A-8C).