TGFB1 can reportedly be synthesized and secreted by tumor cells.[33] We further examined the level of TGFB1 in ICC cell culture supernatants by ELISA and found that cPKM‐WT increased the level of TGFB1 but cPKM‐Δ111–115 did not (Figure 4D). Here, CKM is linked to intrahepatic cholangiocarcinoma.