To exclude the possibility that the efficacy of LNPs in the orthotopic model was influenced by cPKM silencing in a portion of intrahepatic macrophages because of the uptake of nanoparticles, we estimated the level of cPKM in Kupffer cells using qRT‐PCR and found it to be significantly lower in Kupffer cells than in ICC cells (Figure S6K, Supporting Information). The gene discussed is CKM; the disease is intrahepatic cholangiocarcinoma.