Moreover, Western blot analysis also showed that the expression of hepatic stellate cell activation markers (α‐SMA, fibronectin, and COL1A1) decreased after co‐culture with KD‐cPKM ICC cells and increased after co‐culture with OE‐cPKM ICC cells (Figure 2G). The gene discussed is ACTA1; the disease is intrahepatic cholangiocarcinoma.