To investigate whether cPKM exerts its biological functions in ICC cells by regulating IGF2BP2 and miR‐199a‐5p, we transfected wild‐type cPKM plasmids and cPKM plasmids with an IGF2BP2 binding site mutation (cPKM‐Δ111–115) or miR‐199a‐5p binding site mutation (cPKM‐ΔmiR) or both [cPKM‐Δ(111–115+miR)] into HCCC‐9810 cells, respectively. Here, IGF2BP2 is linked to intrahepatic cholangiocarcinoma.