We further verified the expression levels of cPKM in cell lines using qRT‐PCR, and the results showed that all the tested ICC cell lines (HCCC‐9810, RBE, and HuCC‐T1) had significantly upregulated cPKM expression than human intrahepatic bile duct epithelial cells (HIBEpiC, Figure 1L). The gene discussed is CKM; the disease is intrahepatic cholangiocarcinoma.