In view of our omics results, we found that the inflammatory response was one of the main features of endothelial reperfusion injury following ischemia, and TNF pathway was one of the most enriched inflammatory signaling pathways, so we determined the effect of intravenous injection of adalimumab, a TNF‐α neutralizing antibody used in clinic, on mice with cerebral ischemia and reperfusion. The gene discussed is TNF; the disease is brain ischemia.