Similar with the results observed in mouse model for intrahepatic cholestasis brought on by ANIT (Fig. 2E-H), RT-PCR and Western blotting analysis showed that both FGF19ΔKLB and FGF19WT significantly inhibited the mRNA and protein levels of hepatic Cyp7a1 (the rate-limiting enzyme in the classical pathway) and Cyp27a1 (catalyzing BA biosynthesis in the alternative pathway), without alter Cyp8b1 and Cyp7b1 (Fig. 5C-K). Here, CYP8B1 is linked to intrahepatic cholestasis.