We also detected epithelial–mesenchymal transition (EMT)-related proteins (Fig. 2M–O), and found that the expression levels of Vimentin and N-cadherin were significantly decreased, while that of E-cadherin was increased; thus suggesting that RILP overexpression significantly restrains the metastatic potential of osteosarcoma cells. This evidence concerns the gene CDH1 and osteosarcoma.