TP53 and neoplasm: Interestingly, whole-exome sequencing (WES) of the EAC PDOs cultured in NorHA hydrogels of different stiffness (100 or 1000 Pa) or Matrigel revealed the presence of a TP53 gene Pro72Arg (rs1042522) single-nucleotide polymorphism, which is located in the p53 proline-rich domain and has been associated with increased tumor metastasis (Supplemental Figure 6F) (50).