Because our transcriptomic analysis revealed upregulated pathways of chronic inflammatory diseases including inflammatory bowel disease (IBD) in USP47-deficient Tregs (Figure 2B), we assessed the colonic inflammatory responses of Usp47+/+Foxp3-Cre and Usp47fl/flFoxp3-Cre mice using a dextran sodium sulfate–induced (DSS-induced) acute colitis model. Here, USP47 is linked to colitis.