To confirm the importance of YTHDF1 in the defective suppressive functions of USP47-deficient Tregs, we performed IBD induction and tumor inoculation using 6-week-old WT mice, Usp47fl/flFoxp3-Cre mice, and Usp47fl/flYthdf1fl/flFoxp3-Cre mice. The gene discussed is YTHDF1; the disease is inflammatory bowel disease.