We transiently transfected G292 (osteosarcoma) and PC3 (prostate adenocarcinoma) cells with the FGF1_pcDNA3.1 vector or the empty pcDNA3.1 control vector (Fig. S6) and performed apoptosis experiments using the same stress inducers as in the experiments with p53-positive cell lines. Here, TP53 is linked to osteosarcoma.