While recurrent FLT3 mutations are frequently known to represent secondary and accelerating AML mutations15,33,66, our reanalysis of 58 published FLT3D835Y-mutated AML patient samples15,45 demonstrated that this FLT3 mutation frequently is clonal and, in some cases, also might be the initiating mutation or secondary only to a CH mutation. This evidence concerns the gene FLT3 and acute myeloid leukemia.