Viral infection and efficient biotin ligation were confirmed in whole cell lysates derived from NHDFs via immunoblot using HRP-conjugated streptavidin and antibodies directed against HCMV pUL44 and TurboID (Fig 2C). Similar results were obtained using whole cell lysates derived from CD34+ HPCs (Fig 2D). The resulting interacting proteins for all three in vitro models were purified via streptavidin bead-based precipitation, and analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). This evidence concerns the gene CD34 and viral infectious disease.