To delineate the regulation of the NF-κB pathway by TNIP1 in diabetes, as TNIP1 is an A20 binding protein, we first used a coimmunoprecipitation assay in HRMECs to detect the direct interaction among TNIP1, A20 (Supplemental Figure 6E), and IKKγ (Supplemental Figure 6F). This evidence concerns the gene PROS1 and diabetes mellitus.