Our findings are in line with another study that showed that 15 days of HDM exposure caused increased time-of-day effects on airway hyperresponsiveness in WT mice (maximal at the active phase/dark cycle) but not in Rev-erbα KO mice, suggesting a potential involvement of muscarinic receptors (Chrm1 and Chrm3).18 This evidence concerns the gene CHRM1 and airway hyperresponsiveness.