ARF6 and cancer: In cancer cells, it has been shown that activation of ARF6 (ADP-Ribosylation Factor 6), ADP Ribosylation Factor 1 (ARF1), and small GTP-binding proteins lead to phosphorylation of the myosin side chain of myosin and contraction of actomyosin, permitting MV to detach from the membrane (Nabhan et al., 2012).