Compared with untransduced T cells (UTDT), both CAR T cells mediate significant NHL cell lysis capacity, while CD19-s47-CAR T cells mediated more potent killing function (Fig. 4D, Fig. S2C), without significantly killing healthy donor-derived CD34+ stem cells compared to parental CD19-CAR T cells (Fig. 4E). This evidence concerns the gene CD19 and non-Hodgkin lymphoma.