In one case, Gouin et al. incorporated ST (Visium) with single-nucleus RNA-seq and spatial proteomic analysis on bladder cancer and classified an epithelial subtype (expressing Cadherin 12, catenin, and other epithelial markers), which was closely related to the therapeutic response in which Cadherin 12-enriched tumors showed a superior reaction to immune checkpoint therapy compared with neoadjuvant chemotherapy 52. This evidence concerns the gene CDH12 and urinary bladder cancer.