In this study, by crossing Srsf1Floxed/Floxed(Srsf1F/F) mice with Stra8-Cre mice to generate mutant mice with specific deletion of the Srsf1 gene in male germ cells, we found that SRSF1 knockout caused complete infertility and germ cells were drastically lost during spermatogenesis. The gene discussed is SRSF1; the disease is Infertility.