To further investigate the effect of GLDC on prostate cancer metastasis, we downregulated the expression of GLDC by transfecting siRNA into DU145 and LNcap cells (Figure 7A, Supplementary Figure 2A), and the results showed that the migration and invasion abilities were significantly decreased in the si-GLDC group compared with the control group (Figure 7B, C, Supplementary Figure 2B). This evidence concerns the gene GLDC and Familial prostate cancer.