Figures 3E, F show that inhibition of trans-infection by GPI-scFv X5 occurred when CEMss-CCR5-GPI-scFv X5 cells, but not CEMs-CCR5 or CEMss-CCR5-GPI-scFv AB65 cells, were co-cultured with iDCs or mDCs containing captured HIV-1 virions. These results were confirmed in two other independent experiments using iDCs and mDCs derived different donors (Figure S3). Here, CCR5 is linked to infection.