In the present study, we demonstrated that after co-culturing iDCs or mDCs with captured HIV-1 and GPI-scFv-transduced CD4+ cell lines as well as human primary CD4+ T cells, the anti-HIV-1 GPI-scFv X5 inhibitor almost completely blocks trans-infection of the target cells (Figures 2–4). This evidence concerns the gene CD4 and infection.