As apoptosis is closely related to cell cycle in cancer cells, we followed to utilize Annexin V/PI staining assay to show that with the increase of LTe2 concentration, the ability to promote apoptosis of MM cells was enhanced (p=0.0000, p=0.0007, p=0.0020, p=0.0000) (Figures 3B, C). This evidence concerns the gene ANXA5 and Miyoshi myopathy.