According to some studies, declining CD4+ T cell counts can contribute to liver cancer development (28, 29), whereas our results demonstrated that ANKRD27, AP3M1, BCL10, CTSC, HPS1, HPS4, PPT1, RAB7A, TPP1, and RAMP3 had a negative relation to immune infiltration of CD4 T cells using the EPIC algorithm. This evidence concerns the gene ANKRD27 and liver cancer.