MicroPET imaging in the PANC-1 tumor model for competitive TGFβ blockade study was performed with unlabeled DOTA-P144 pretreatment (100 times the mass dose of [68Ga]Ga-P144) for 10 min before the injection of PET tracer [68Ga]Ga-P144 to examine and verify the targeting specificity of P144 carrier against TGFβ. This evidence concerns the gene TGFB1 and neoplasm.