Notably, high expression levels of NUSAP1 were positively correlated with various immune checkpoint molecules, including CD274 (PD-L1), CD276, TIGHT, PDCD1 (PD-1), CTLA4, LAG3, IDO1 in BRCA, GBM, KICH, KIRC, KIRP, LGG, PAAD, LUAD, STAD, and THCA. Here, LAG3 is linked to glioblastoma.