Additionally, patients with high NUSAP1 expression in KICH, KIRC, KIRP, LUAD, LUSC, PARD and SKCM were more prone to lymph node metastasis (N status), and NUSAP1 levels positively correlated with distant metastases (M status) in patients with KIRC, KIRP, LIHC, and LUAD. This evidence concerns the gene NUSAP1 and metastatic malignant neoplasm in the lymph nodes.