PBX3 and neoplasm: To investigate the role of PBX3 in glucose metabolism in tumor cells, we first analyzed the effect of knocking down PBX3 on tumor cell viability and colony formation potentials using two shRNA expression vectors targeting different sites of PBX3(Supplementary Figure S1A, B). Knocking down PBX3 significantly suppressed HCT116 cell viability as well as their colony formation potential (Figure 1A, B).