ENTREP3 and intrahepatic cholangiocarcinoma: To probe the effect of COTE1 overexpression on proliferation of ICC cells, we transfected the recombinant vector containing COTE1 into RBE and HuCCT1 cells and confirmed the overexpression of COTE1 by qRT-PCR (P < 0.05; Figures 2(a) and 2(b)).