B16–F10 is a highlyaggressive murine cancer model and is widely used for either subcutaneousor metastatic models of melanoma.53−55 B16 tumors are knownto downregulate class I MHC to limit recognition by cytotoxic T cells.56,57 We chose to target two cell-studied tumor associated antigens expressedby B16 tumors, Trp1 and gp100.58−60. This evidence concerns the gene PMEL and cancer.