Subsequently, Hk3-OE and NC GL261 cells were injected into the brains of C57BL/6 N mice through cranial guide screws, as shown in Fig. 6B. In these models, the Hk3-OE group showed a significant increase in intracranial tumor volume compared to the NC group (Fig. 6C-D). The gene discussed is HK3; the disease is neoplasm.