Our present study demonstrates that HK3 can regulate glioma microenvironment by elevating the infiltration level of M2 macrophage, neutrophil, and various subtypes of activated memory CD4+ T. In addition, HK3 expression is significantly elevated along with the tumor grade and is the highest in GBM samples compared with Grade 2 and 3 samples and can predict unfavorable prognosis of glioma patients in vitro and in vivo. The gene discussed is CD4; the disease is central nervous system cancer.