Based on the results of the methylation chip of Illumina Infinium 450K detection and bioinformatic analysis, five hypermethylated genes, ZNF471, SND1, SPOCK1, FBLIM1, and OTX1, were selected for further investigation in the colorectal adenoma, CRC, and control normal tissues in our study. Here, OTX1 is linked to colorectal carcinoma.