To investigate the relevance of these activities on tumor growth, MDA-MB-231/Luc EYA4-depleted cells complemented with either EYA4 S/T phosphatase deficient mutant or Y phosphatase deficient mutant were injected into the tail-vein and monitored by in vivo imaging for 4 weeks using the luciferase reporter. This evidence concerns the gene EYA4 and neoplasm.