PYCR1 and colorectal neoplasm: To determine whether PYCR1 can regulate ELK4-mediated transcription under hypoxia in colorectal tumor cells, the RNA-seq analysis was performed in WT rPYCR1- and rPYCR1 Y135F-expressing HCT116 cells under hypoxia, which indicated 656 genes were upregulated and 373 genes were downregulated by rPYCR1 Y135F expression (Supplementary Data file 1).