To determine how hepatic deficiency of Wtap increases protein stability of GRB2 and ERK1/2, we analyzed our previous RNA-Seq data (GSE168850) (7) and noticed that downregulated genes were associated with the proteasome (Fig. 5A), and 28 proteasome-associated genes were downregulated (Fig. 5B), which was confirmed by RT–quantitative PCR (qPCR) (Fig. S3). This evidence concerns the gene MAPK3 and hyperinsulinemic hypoglycemia, familial, 4.